Archive for the ‘DMSO’ Category

Dimethyl Sulfoxide (DMSO)

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Posted 11 Nov 2011 — by James Street
Category DMSO

In August 1995, Dr. Julian Whitaker, M.D., relayed his own experience with DMSO, when a basal cell carcinoma (about the size of a dime) appeared on his ear. A dermatologist recommended surgical removal of the cancerous portion and a skin graft replacement. Instead, Dr. Whitaker made a paste from shark cartilage, vitamin C, and DMSO and applied the mixture to the lesion daily. Within 3.5 weeks, the basal cell had completely disappeared. Stanley Jacob, M.D., professor at the Oregon Health Sciences University (Portland) suspected DMSO was the hero, although Dr. Whitaker has confidence in the full formula (Whitaker 1995).

The Sealy Center for Molecular Sciences reported that DMSO, administered either before or 15 minutes after TNF-alpha, blocked 80% of NF-kB. By suppressing TNF-alpha and NF-kB, DMSO broke an inflammatory cascade that otherwise terminates in an onslaught of potentially damaging cytokines (Vlahopoulos et al. 1999).

DMSO is an excellent transporter of other therapies into cancerous cells. In fact, many offshore cancer clinics consider it the standard for all patients who are undergoing various therapies.

Cytotoxic effects of dimethyl sulphoxide (DMSO) on cochlear organotypic cultures

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Posted 17 Jun 2011 — by James Street
Category Apoptosis, DMSO
Hear Res. 2008 Feb;236(1-2):52-60. Epub 2007 Dec 15.

Source

Center for Hearing and Deafness, State University of New York at Buffalo, 137Cary Hall, Buffalo, NY 14214, USA.

Abstract

The amphipathic molecule dimethyl sulphoxide (DMSO) is a solvent often used to dissolve compounds applied to the inner ear; however, little is known about its potential cytotoxic side effects. To address this question, we applied 0.1-6% DMSO for 24h to cochlear organotypic cultures from postnatal day 3 rats and examined its cytotoxic effects. DMSO concentrations of 0.1% and 0.25% caused little or no damage. However, concentrations between 0.5% and 6% resulted in stereocilia damage, hair cell swelling and a dose-dependent loss of hair cells. Hair cell damage began in the basal turn of the cochlea and spread towards the apex with increasing concentration. Surprisingly, DMSO-induced damage was greater for inner hair cells than outer hair cell whereas nearby supporting cells were largely unaffected. Most hair cell death was associated with nuclear shrinkage and fragmentation, morphological features consistent with apoptosis. DMSO treatment induced TUNEL-positive staining in many hair cells and activated both initiator caspase-9 and caspase-8 and executioner caspase-3; this suggests that apoptosis is initiated by both intrinsic mitochondrial and extrinsic membrane cell death signaling pathways.

PMID:
18207679
[PubMed - indexed for MEDLINE]
PMCID: PMC2262105

Free PMC Article

DMSO protects against adriamycin-induced tissue necrosis

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Posted 17 Jun 2011 — by James Street
Category Adriamycin, DMSO, doxorubicin

Luis Lebredo M.D.a, Rosemary Barrie M.S.a and Eugene A. Woltering M.D.Corresponding Author Contact Information, a

aDivision of Surgical Oncology, Department of Surgery, Oregon Health Sciences University, Portland, Oregon 97201 USA

Received 7 December 1990.
Available online 9 February 2004.

 

Abstract

Inadvertent extravasation of Adriamycin (Adria) can result in severe tissue necrosis. The mechanism of this tissue damage is believed to be the release of free radicals into the tissue. Topical applications of dimethylsulfoxide (DMSO) used after Adria extravasation have been shown to decrease ulcer size. This may be due to DMSO’s ability to scavenge free radicals. However, effective topical therapy requires prompt recognition of extravasation, which is often difficult. We hypothesized that the delivery of Adria in low concentrations DMSO would reduce Adria-induced ulcer size and ulcer incidence caused by Adria extrasasation. To test this hypothesis, 180, male Sprague-Dawley rats were randomly allocated into three treatment groups of 60 each. All three groups received intradermal injections of Adria (1 mg) diluted in 0.5 cc of saline (Group 1), 10% DMSO (Group 2), or 20% DMSO (Group 3). Rats were observed for 4 weeks. Ulcer incidence (%) and size of ulcers (mm2) were assessed over time. Area of skin ulceration was calculated as the product of the two greatest diameters. Statistical evaluation of the differences in incidence and ulcer size between Group 1 and Groups 2 or 3 were evaluated using analysis of variance. Delivery of Adriamycin in 10 or 20% DMSO resulted in a statistically significant (P < 0.001) decrease in the incidence of ulceration caused by intentional Adria extravasation.

star, openSupported in part by the Medical Research Foundation of Oregon, and the American Cancer Society Clinical Fellowship 00162.
Corresponding Author Contact InformationTo whom reprint requests should be addressed at Division of Surgical Oncology L-224A, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, Portland OR 97201-3098.

Copyright © 1992 Published by Elsevier Inc.

Prevention of cytotoxic drug induced skin ulcers with dimethyl sulfoxide (DMSO) and α-tocopherole

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Posted 17 Jun 2011 — by James Street
Category alpha tocopherol, antioxidants, Chemotherapy, DMSO, Lung Cancer



Christian U. LudwidCorresponding Author Contact Information, a, Hans-Rudolf Stolla, Reto Obristla and Jean-Paul Obrechta

aDivision of Oncology, Department of Internal Medicine of the University, Kantonsspital, Basel, Switzerland

Accepted 11 September 1986.
Available online 12 May 2004.

 

Abstract

Accidental subcutaneous extravasation of several antineoplastic agents may provoke skin ulcerations for which there has been no simple and effective treatment. Since January 1983 we have treated all patients in our institution sustaining extravasation by a cytotoxic drug with a combination of DMSO and α-Tocopherole. During the first 48 hr after extravasation a mixture of 10% α-Tocopherole acetate and 90% DMSO was topically applied. The bandage was changed every 12 hr. So far eight patients with extravasation of an anthracycline or Mitomycin were treated on this protocol. No skin ulceration, functional or neurovascular impairment occured in any of these patients. The only toxic effect observed by this treatment was a minor skin irritation. The combination of DMSO and α-Tocopherole seems to prevent skin ulceration induced by anthracyclines and Mitomycin.

star, openPresented in Part at the IV World Conference on Lung Cancer, 25–30 August 1985, Toronto, Canada.